Neuroprotective effects of steroids against Aβ25-35-induced decrease on neurite outgrowth in B104 cultured cells. Histograms of the length of the longest neurite per cell correspond to the pool of 3-4 independent experiments. An average of 30-40 longest neurites was counted per treatment group and per day. (A) Effects of ent-PREGS (ent-PS, 5 μM) and PREGS (PS, 5 μM) alone or in presence of Aβ25-35 (20 μM). Two-way ANOVA: F(5,664) = 79.5, p < 0.001 for the steroid treatment; F(2,664) = 26.1, p < 0.001 for the day; F(10,664) = 2.1, p > 0.05 for the treatment × day interaction. (B) Effects of ent-DHEAS (ent-DS, 5 μM) and DHEAS (DS, 5 μM) alone or in the presence of of Aβ25-35 (20 μM). Two-way ANOVA: F(5,585) = 40.3, p < 0.001 for the steroid treatment; F(2,585) = 20.8, p < 0.001 for the day; F(10,664) = 1,4, p > 0.05 for the treatment × day interaction. * p < 0.05, *** p < 0.001 vs. control; ## p < 0.01, ### p < 0.001 vs. Aβ25-35-treated cells. Fisher's PLSD test.