Skip to main content
. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Nat Neurosci. 2014 Jul 6;17(8):1046–1054. doi: 10.1038/nn.3757

Figure 4.

Figure 4

Fezf2 promotes glutamatergic and inhibits GABAergic neurotransmitter pathways. (a) ChIP-seq trace shows that 3xFlag-Fezf2 binds specifically to the promoters of Vglut1 (Slc17a7) and Gad1 but not Vglut2 (Slc17a6). (b) RNA-seq analysis shows the effect of 3xFlag-Fezf2 overexpression on these three genes in neural stem cells in vitro. (c) Inducible expression of single-copy Fezf2-IRES-GFP is show by immunoblotting and fluorescence microscopy. Uncropped original immunoblots are shown in Supplementary Figure 10. (d) Schematic representation of directed differentiation protocol used to instruct embryonic stem cell (ES) differentiation into mixed populations of cortical neurons23. (e) RNA-seq analysis showing the effect of Fezf2 expression on Vglut1, Vglut2 and Gad1 in ES-derived neurons. Clones used for RNA-seq were from the differentiation of n = 2 independently generated iGFP and n = 2 iFIG lines. the error bars represent 95% confidence intervals for the Cuffdiff2 model expression estimate as defined in Methods and described in Trapnell et al., 2013.