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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Mol Cancer Ther. 2014 Jul 31;13(10):2303–2314. doi: 10.1158/1535-7163.MCT-13-1005

Figure 6.

Figure 6

ICG-001 directly regulates change in SKP2 and CDKN1A expression. (A) Chromatin immunoprecipitation results for CBP association with SKP2, CDKN1A and CDC25A regulatory elements in AsPC-1 cells following 1 hour treatment with vehicle or 10 μM ICG-001. Data were normalized to input DNA and are presented as fold change of CBP/IgG. Representative experiments of three replicates are shown. (B-E) Time course study of AsPC-1 cells treated with vehicle or 10 μM ICG-001 measuring (B) SKP2 and (C) CDKN1A gene expression by qPCR, (D) SKP2, MYC, p21 and tubulin protein expression by western blot and (E) MYC gene expression by qPCR. All qPCR values were normalized to ACTB and shown relative to 0 hour. Representative experiments from at least two replicates are shown for all qPCR and western blots. *P<0.05, **P<0.01, ***P<0.001, ns=not significant.