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. 2014 Sep 26;14:236. doi: 10.1186/s12866-014-0236-0

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© Britta et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Differential interference contrast microscopy (DIC) and fluorescence microscopy with Nile Red staining of Leishmania amazonensis promastigotes without treatment (A, B) and treated with concentrations that corresponded to the IC ₅₀ (C-F) and IC ₉₀ (G-L) of BZTS for 48 h. In the treated promastigotes, the images suggest the concentration-dependent accumulation of lipid-storage bodies in the cytoplasm. (G, I, K) The parasites that were treated with the IC₉₀ of BZTS were completely modified. Scale bar = 10 μm.