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Color deconvolution in images of epithelial cells with different staining patterns: a) p16+/Ki67+, b) p16−/Ki67+, c) p16+/Ki67−, and d) p16−/Ki67− . Respective columns labeled DAB and FastRed display localization of individual stains after deconvolution demonstrating that only FastRed and Haematoxylin images are suitable for nuclear segmentation.
