Figure 5. RGS5⁺ HSCs participate in the response to acute hepatic injury.

Anti-GFAP and anti-β-gal immunofluorescence were used to localize HSCs in acutely injured liver tissue. A–F. Low magnification images. G–I. High magnification of Rgs5^LacZ/LacZ A,D Uninjured liver tissue from Rgs5^+/+ and Rgs5^LacZ/LacZ mice show sparse HSCs distributed throughout the liver. High magnification in uninjured Rgs5^LacZ/LacZ shows HSCs are GFAP⁺ and β-gal⁺. B,E. At 48 hours post injury, HSCs are concentrated in the necrotic foci surrounding the central veins. β-gal⁺ cells (E,H) are associated with GFAP⁺ cells. C,F At 96 hours post injury, HSCs are tightly clustered at the foci of injury. I. β-gal⁺ cells are GFAP⁺. All scale bars are 100 µm.