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. 2014 Oct 7;9(10):e108505. doi: 10.1371/journal.pone.0108505

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© 2014 Bahrami et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Rgs5^LacZ/LacZ mice were chronically injected with CCl₄ (or oil), twice weekly for 4 weeks. RNA was isolated from whole liver and analyzed by qPCR for expression of Rgs5 and HSC activation markers. A. Chronic CCl₄ treated Rgs5^LacZ/LacZ dab labeled with anti-SMA shows activated HSCs. B. Rgs5^LacZ/LacZ liver immunolabeled with α-β-gal and α-GFAP antibodies. GFAP⁺ HSCs are visible along fibrotic septa bridging the portal veins. β-gal⁺ HSCs are visible in Rgs5^LacZ/LacZ (B) around the fibrotic septa. Scale bars are 100 µm. C. qPCR of Rgs5^+/+ liver RNA shows Rgs5 is up-regulated in chronic CCl₄ injury. Collagen 1α expression is elevated and multiple HSC activation markers (PDGFRα, PDGFRβ, and SMA) are increased relative to oil-injected mice. Data is normalized to oil injected mice. n = 6; error bars = SEM; * = p<.05.