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. 2014 Oct 7;9(10):e108672. doi: 10.1371/journal.pone.0108672

Table 2. Primers used in polymerase chain reaction amplification and sequencing of the mitochondrial COI gene and the 18S and 28S rRNA genes.

Primer name Sequence Application References
COI
C1-J-1718 Forward primer 5′-GGAGGATTTGGAAATTGATTAGTTCC-3′ PCR amplification & sequencing Simon et al. [30]
COI REVA Reverse primer 5′-GATAAAACGTAATGAAAATGAGCTAC-3′ PCR amplification & sequencing Gotoh et al. [8]
C1-J-1718-stig Forward primer 5′-GGAGGTTTTGGTAATTGGTTAATCCC-3′ PCR amplification & sequencing This study
COI REVA-stig Reverse primer 5′-GAAAGAACATAATGAAAATGAGCAAC-3′ PCR amplification & sequencing This study
18S
18S-1F Forward primer 5′-ACCGCGAATGGCTCATTAAATCAGTT-3′ PCR amplification & sequencing This study
18S-2F Forward primer 5′-TGGCCTCTGAGCCGACGATGTAT-3′ Sequencing This study
18S-2R Reverse primer 5′-ACCCCATAGGTTCGACTGAAATC-3′ Sequencing This study
18S-5R Reverse primer 5′-TCCAATAGATCCTCGTTAAAGGAT-3′ Sequencing This study
18S-8R Reverse primer 5′-TCTCGTTCGTTATCGGAATTAAC-3′ Sequencing This study
18S-9F Forward primer 5′-AGCTTCCGGGAAACCAAAGTTT-3′ Sequencing This study
18S-9R Reverse primer 5′-AGGGCATCACAGACCTGTTATT-3′ Sequencing This study
18S-10F Forward primer 5′-AGTTGGTGGAGTGATTTGTCTGGT-3′ Sequencing This study
18S-10R Reverse primer 5′-ACAAAGGGCAGGGACGTAATCAA-3′ PCR amplification & sequencing This study
28S
28v-5′ Forward primer 5′-AAGGTAGCCAAATGCCTCATC-3′ PCR amplification & sequencing Hillis and Dixon [31], Palumbi [32]
28jj-3′ Reverse primer 5′-AGTAGGGTAAAACTAACCT-3′ PCR amplification & sequencing Hillis and Dixon [31], Palumbi [32]