Table 2. Proteins from roots of the TSA-792 genotype of Theobroma cacao identified by mass spectrometry.
| Spot n°a | Accession n° | Protein Name | Theoretical MW(KDa)/pI | Biological process | Fold change b |
| Upregulated | |||||
| 1 | XP_002513404.1 | Eukaryotic Translation Elongation Factor | 99.23/5.79 | Protein biosynthesis | ↑2.0 |
| 6 | AAF04851.1 | Alcohol Dehydrogenase A | 41.64/6.57 | Cellular metabolic process | ↑7.5 |
| 7 | ABW21688.1 | Enolase | 47.94/5.48 | Glycolysis | ↑3.3 |
| ACJ11711.1 | UDP-Glucose Pyrophosphorylase | 51.28/5.51 | Metabolic process | ↑3.3 | |
| 11 | AAF04851.1 | Alcohol Dehydrogenase A | 41.64/6.57 | Cellular metabolic process | ↑9.5 |
| XP_002528593.1 | Fructose Bisphosphate Aldolase | 40.92/6.53 | Pentose-phosphate shunt/Glycolysis | ↑9.5 | |
| 12 | XP_002519823.1 | Acetylornithine Deacetylase | 52.74/5.18 | Proteolysis | ↑2.0 |
| 13 | AAL92037.1 | Peroxidase | 37.15/4.43 | Response to oxidative stress | ↑2.1 |
| 18 | ABA46792.1 | Triosephosphate Isomerase | 27.21/5.4 | Pentose-phosphate shunt/Glycolysis/Gluconeogenesis | ↑3.0 |
| 19 | ABR68691.2 | Ascorbate Peroxidase | 27.56/5.42 | Response to oxidative stress | ↑2.9 |
| 20 | XP_002513374.1 | Proteasome Subunit Alpha Type-2-B | 25.64/5.34 | Defense response to bacterium/Ubiquitin-dependent protein catabolic process | ↑2.4 |
| 21 | XP_002530662.1 | 3-Isopropylmalate Small Subunit | 26.81/5.69 | Leucine biosynthetic process | ↑2.3 |
| 22 | AAX86687.1 | Nonsymbiotic Hemoglobin | 18.35/9.07 | Oxygen transport/Nitrogen fixation/Response to stress | ↑5.3 |
| Downregulated | |||||
| 2 | XP_002511690.1 | Transketolase | 80.61/6.34 | Energy reserve metabolic process | ↓6.4 |
| 3 | YP_004237280.1 | ATPase Subunit 1 | 55.26/5.74 | ATP synthesis | ↓6.5 |
| 4 | NP_565040.2 | Alanine Aminotransferase | 59.27/5.97 | Response to hypoxia/biosynthetic process1-aminocyclopropane-1-carboxylate | ↓2.1 |
| 5 | AAC67586.1 | Pyrophosphate-DependentPhosphofructokinase Beta Subunit | 61.63/5.8 | Glycolysis/Phosphorylation | ↓2.9 |
| 8 | XP_002522953.1 | DNA Binding Protein | 45.83/5.65 | Nucleotide binding | ↓2.9 |
| NP_001235426.1 | Mitogen-Activated Protein Kinase | 48.97/5.51 | Protein amino acid phosphorylation/Cell division | ↓2.9 | |
| 9 | XP_002519309.1 | S-Adenosyl methionine Synthetase | 42.97/5.5 | One-carbon metabolic process | ↓3.5 |
| 10 | XP_002512941.1 | Diaminopimelate Decarboxylase | 58.76/7.05 | Lysine biosynthetic process via diaminopimelate | ↓3.3 |
| 14 | ADD51354.1 | Anthocyanidin Reductase | 36.27/6.05 | Flavonoid biosynthetic process | ↓8.0 |
| ACJ11738.1 | Cytosolic Malate Dehydrogenase | 36.57/6.67 | Cellular carbohydrate metabolic process | ↓8.0 | |
| 15 | CAC86003.1 | Aspartic Proteinase | 59.75/4.86 | Proteolysis/Lipid metabolic process | ↓2.0 |
| 16 | ACJ11750.1 | Lactoylglutathione Lyase-Like Protein | 32.5/5.64 | Carbohydrate metabolic process | ↓3.3 |
| 17 | BAJ10727.1 | Protein Transport Protein Sec13 | 32.71/5.65 | Membrane budding | ↓2.7 |
| Unique to one of | the treatments | ||||
| 23 | AAD11255.1 | Class I Chitinase | 34.84/5.06 | Chitin catabolic process/Defense response/Cell wall | N |
| 24 | YP_004237280.1 | ATP Synthase F1 Subunit 1 | 55.26/5.74 | ATP synthesis | N |
| 25 | XP_002533858.1 | 26S Proteasome Non-ATPase Regulatory | 44.54/4.2 | Protein catabolic Process proteolysis | N |
| 26 | ACJ11728.1 | G lyceraldehyde-3-PhosphateDehydrogenase | 36.67/8.12 | Reductive pentose-phosphate cycle/Glycolysis | N |
| 27 | XP_002525377.1 | Carbonic Anhydrase | 28.07/5.16 | Carbon utilization | N |
| 28 | XP_002523700.1 | Glyoxalase I | 26.8/8.42 | Response to stress | N |
| 29 | AAV41231.1 | Trypsin Inhibitor | 23.92/5.92 | Negative regulation of endopeptidase activity | N |
a
Number of spots correspond to the numbers in pictures 2-D gel in Fig. 5. b Proteins differentially expressed in roots of the genotype of T. cacao TSA-792 subjected to 96 h of flooding. Proteins with abundance increased (↑) or decrease (↓) or (N) unique to one of the treatments (control and flooding) in response to flooding. The number represents changes in the rate of protein abundance in the treatment compared to control flooded. e-value = 0.0.