TABLE 6.

Validation of microarray results by real-time PCR

mRNA induction for 17 genes, found up-regulated by microarray, after a 6-h treatment with PACAP (100 nM), forskolin (25 μM), dbcAMP (1 mM), or NGF (100 ng/ml). Genes were classified in ascending order of regulation by PACAP obtained by real-time PCR.

Gene	Control	S.E.M.	PACAP	S.E.M.	Forskolin	S.E.M.	dbcAMP	S.E.M.	NGF	S.E.M.
Gata2	1.19	0.08	1.06	0.11	3.21	0.32	1.78	0.41	0.69	0.05
Nrp1	1.23	0.25	1.08	0.13	0.41	0.00	0.42	0.03	5.26	0.62
Pac1	1.03	0.11	1.23	0.13	0.77	0.05	0.66	0.06	7.20	0.81
Anx2	1.06	0.05	1.35	0.16	1.29	0.04	1.70	0.19	1.54	0.37
Homer2	1.08	0.06	2.18	0.13	2.08	0.12	1.10	0.37	1.56	0.21
Por	0.97	0.06	2.46	0.28	2.09	0.10	2.58	0.25	3.52	0.56
Akr1b8	1.06	0.05	2.73	0.36	1.80	0.19	4.06	1.49	3.31	0.96
Vil2	1.10	0.11	2.86	0.26	2.50	0.09	3.40	0.47	2.31	0.26
Hspb8	1.06	0.05	2.95	0.16	2.24	0.10	1.25	0.42	1.88	0.19
Egr1	1.29	0.30	4.04	0.36	2.30	0.12	4.22	0.66	48.60	13.83
Glrx	1.10	0.06	6.42	0.33	3.57	0.22	4.19	0.26	1.14	0.08
Ptp4a1	1.21	0.16	6.64	0.45	4.53	0.15	6.61	1.78	2.85	0.39
Gas1	0.87	0.14	7.45	1.63	6.51	0.66	8.62	3.09	0.57	0.20
Azin	1.11	0.08	11.44	2.33	8.69	0.44	3.93	1.36	1.37	0.14
Odc	1.01	0.06	12.76	1.75	5.69	0.62	7.98	1.89	7.19	1.45
Ier3	1.11	0.07	33.88	3.58	14.34	1.02	15.80	1.21	3.17	0.77
Rgs2	1.35	0.10	76.70	12.83	25.66	3.10	29.31	4.61	9.67	1.24

Gata2, GATA binding protein 2; Nrp1, neuropilin 1; Pac-1, adenylate cyclase activating polypeptide 1 receptor 1; Anxa2, annexin A2; Homer2, homer homolog 2 (Drosophila); Por, P450 (cytochrome) oxidoreductase; Akr1b8, aldo-keto reductase family 1, member B8; Vil2, villin 2; Hspb8, 22-kDa heat shock protein 8; Egr1, early growth response 1; Glrx, glutaredoxin; Ptp4a1, protein tyrosine phosphatase 4a1; Gas1, growth arrest specific 1; Azin1, antizyme inhibitor 1; Odc1, ornithine decarboxylase, structural 1; Ier3, immediate early response 3; Rgs2, regulator of G-protein signaling 2.