Figure 3. Autophosphorylation of Cph1 at various temperatures.
(A) Autoradiogram (above) and Coomassie-stained blot (below) of Cph1 holoprotein. Samples were irradiated either with FR or R as indicated and incubated with γ−32P ATP in darkness at the indicated temperatures (°C). (B) Mean phosphorylation intensities of three experiments ± SE as shown in (A) are plotted over temperature. The 100% value corresponds to the mean signal of the FR irradiated sample at 20°C. as shown in (A).