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. 2014 Oct 7;9(10):e108776. doi: 10.1371/journal.pone.0108776

Figure 8. 21-BD increases the expression of Na,K-ATPase in MDCK cells.

Figure 8

(A) Protein cell content of the α1 subunit of the Na,K-ATPase of confluent monolayers of MDCK cells grown on filters in control medium (white bar) or treated with different concentrations of 21-BD (red bars) for 48 h; upper part of the figure A shows representative immunoblots of the α1 subunit of the Na,K-ATPase and actin, the lower part the densitometric analysis. (B and C) Na,K-ATPase α1 subunit stained with a fluoresceinated antibody (B, C, white) or Topro (blue) to detect the nuclei.