Table 2. Primer sequences, annealing temperatures and Ensembl transcripts for 23 amplicons of GNAI loci.
| Primer | Sequence (5′ –3′) | Tm (°C) | Trancript |
| Gαi1_ex1_F | GGATTCCCCTGTGCTTGGA | 60 | ENST00000442586 |
| Gαi1_ex1_R | GTTTCCAAACGCCGAGGG | ||
| Gαi1_ex2&3_F | CACACAGAGAGAGACTGGGTG | 60 | ENST00000351004 |
| Gαi1_ex2&3_R | GGTCCTGATAGTTGACAAGCC | ||
| Gαi1_ex4_F | AAGGAAGTTCGCTATTGCC | 60 | ENST00000351004 |
| Gαi1_ex4_R | AATGTGTCAGCCAATTCTGC | ||
| Gαi1_ex5_F | GTTTTGGATGATCTTTATTGGC | 60 | ENST00000351004 |
| Gαi1_ex5_R | TCTCCCAAACATTCTTTTGTCC | ||
| Gαi1_ex6_F | CCCATAAAGTCCTTCTCTCCTTC | 62×1, 61×1, 60×2, 59×2,58×2 | ENST00000351004 |
| Gαi1_ex6_R | CTTGGCAACACCTTCAGCTC | ||
| Gαi1_ex7_F | TGTTCTGAAATGGCAGAAATG | 60 | ENST00000351004 |
| Gαi1_ex7_R | CTGAATTCTTGCCTTAGGGG | ||
| Gαi1_ex8_F | GGAGTCCATGAATGAAACTGTATG | 60 | ENST00000351004 |
| Gαi1_ex8_R | TTTGGTCAAGTCCCAGATGC | ||
| Gαi2_ex1c_F | TCACCCACATCACCGTCTAA | 59 | ENST00000422163 |
| Gαi2_ex1c_R | ACGCGTCCTCTTGCAACTA | ||
| Gαi2_ex1d_F | CGCTGTCCATTGCTCTTCAT | 60 | ENST00000451956 |
| Gαi2_ex1d_R | GCACATGTGAGCATTCAGGT | ||
| Gαi2_ex2_F | AGCTGAAGTGTGACGCTGTG | 58 | ENST00000266027 |
| Gαi2_ex2_R | CTTGGCCAGCCATGAAGG | ||
| Gαi2_ex3&4_F | ATGTGAGAACAGGGTGGCTC | 58 | ENST00000266027 |
| Gαi2_ex3&4_R | GGATTCCCTAGGATGAGACTTG | ||
| Gαi2_ex5_F | CCAAGAATACCCTAGCCTGG | 60 | ENST00000266027 |
| Gαi2_ex5_R | GCAAAGACCAGCAGTGTCC | ||
| Gαi2_ex6_F | CTACCTGAACGACCTGGAGCGTA | 58 | ENST00000266027 |
| Gαi2_ex6_R | CTCTGCTACCCCAGAGGCTG | ||
| Gαi2_ex7&8_F | AAATGGGGTAGAAAGCCTCC | 58 | ENST00000266027 |
| Gαi2_ex7&8_R | TGGTCACCATAGGCTACTTGG | ||
| Gαi2_ex9_F | CTTGCTGCACACGTAGGATG | 58 | ENST00000266027 |
| Gαi2_ex9_R | CGCTTAGTTCTTCCCCAGC | ||
| Gαi2_ex9b_F | GTCCACCTGCTCATTCTCGT | 60 | ENST00000266027 |
| Gαi2_ex9b_R | TGGAACCCAATTCTGTGGAG | ||
| Gαi3_ex1_F | GCAGTTTCCGTGGTGTGAG | 58 | ENST00000369851 |
| Gαi3_ex1_R | GTTCAGGCCTTCCAAGCG | ||
| Gαi3_ex2&3_F | TAGGACCCGTGGTTTTCATC | 60 | ENST00000369851 |
| Gαi3_ex2&3_R | TTGTTGCTTAAATTCATTTCCC | ||
| Gαi3_ex4_F | CTGGCCTGTCAGAAAAGGTC | 60 | ENST00000369851 |
| Gαi3_ex4_R | AAACATTTCCTTAAGTGGGGAC | ||
| Gαi3_ex5_F | TTTGCTATGCACATGGTTGG | 60 | ENST00000369851 |
| Gαi3_ex5_R | AAATTTTACCCTGATTAAGAGATGG | ||
| Gαi3_ex6_F | CATTTCAGTTTAGGGGAAGGTG | 60 | ENST00000369851 |
| Gαi3_ex6_R | TTATTTTCCATTTCCTGGCTAC | ||
| Gαi3_ex7_F | TGAATGCCATTTAGTGCTGC | 60 | ENST00000369851 |
| Gαi3_ex7_R | GCCACTACCACTGAATACTCTCC | ||
| Gαi3_ex8_F | TTGGGTTATGTTCCCTCTCC | 60 | ENST00000369851 |
| Gαi3_ex8_R | CAAGAGACATCACTGTAGCACTATAAC |
Tm: annealing temperature.