Figure 2. Myotoxic activity of Asp49 and Lys49 myotoxins from Bothrops asper, alone or in combination, injected by intramuscular route in CD-1 mice.

Plasma creatine kinase activity was determined 3 h after injection of the indicated amounts of toxins. Control mice received only vehicle (PBS). Each bar represents mean ± SD of five animals. Statistically significant differences (p<0.05) between two groups are indicated by dotted arrow lines. Synergistic action is shown at a total dose of 10 µg in (A) or 20 µg in (B), respectively.