Figure 3. Cytotoxic activity of p-bromophenacyl bromide-modified Asp49 myotoxin (Asp49pb) and Lys49 myotoxin, alone or in combination at 10 µg (A) or 5 µg (B), upon C2C12 myotubes.
The indicated amounts of toxins were added in a total volume of 150 µL. Cytolysis was determined by the release of lactate dehydrogenase to the medium, 3 h after exposure of the cells to the toxins. Each bar represents mean ± SD of triplicate cell cultures. Statistically significant differences (p<0.05) between two groups are indicated by dotted arrow lines.