Figure 6. Cytotoxic activity of a fixed amount of Lys49 myotoxin, alone or in combination with a low amount of Asp49 myotoxin.

In this experiment, Asp49 myotoxin was first incubated for variable periods of time with C2C12 myotubes, and then washed five times, before the addition of Lys49 myotoxin. The indicated amounts of toxins were added in a total volume of 150 µL. Cytolysis was determined by the release of lactate dehydrogenase to the medium 3 h after exposure of the cells to the toxins. Each bar represents mean ± SD of triplicate cell cultures. All values from cultures where the Lys49 myotoxin was added together with Asp49 myotoxin were significantly different (p<0.05) from the value of cultures exposed only to Lys49 myotoxin (indicated by asterisks).