Figure 4. Live-cell imaging of both mutants GFP-CTD^{Δ723 −741} and GFP-CTD^Δ714–722.

COS-1 cells co-expressing either GFP-CTD^{Δ723 −741} (A) or GFP-CTD^Δ714–722 (B), along with the ER/DsRed marker, were subjected to live-cell imaging combined with the in vivo membrane protease protection assay, as described above in Figure 3. The images shown herein are a representative of at least three independent experiments undertaken on separate occasions that were each performed in triplicate (n = 9).