Table 2. Candidate HKGs in geNorm evaluation.
Rank | Temporal Cortex | Frontal Cortex | Hippocampus | Hypothalamus | Total | |||||
Gene | M-value | Gene | M-value | Gene | M-value | Gene | M-value | Gene | M-value | |
1 | ACTB | 0.338 | GAPDH | 0.411 | HPRT | 0.378 | GAPDH | 0.252 | GAPDH | 0.642 |
2 | GAPDH | 0.353 | ACTB | 0.427 | β2M | 0.384 | β2M | 0.271 | HPRT | 0.663 |
3 | β2M | 0.399 | HPRT | 0.473 | ACTB | 0.395 | ACTB | 0.296 | β2M | 0.728 |
4 | HPRT | 0.402 | 18S | 0.474 | GAPDH | 0.420 | HPRT | 0.308 | ACTB | 0.762 |
5 | 18S | 0.563 | β2M | 0.520 | 18S | 0.555 | 18S | 0.383 | 18S | 1.065 |
Ranking of candidate HKGs by geNorm for each brain structure and all structures together as described by the total M-value. Lower M-values indicate more stable genes. A cut-off value of 1.5 was used.