Table 3. Candidate HKGs in NormFinder evaluation.
Rank | Temporal Cortex | Frontal Cortex | Hippocampus | Hypothalamus | Total | |||||
Gene | Stab. Value | Gene | Stab. Value | Gene | Stab. Value | Gene | Stab. Value | Gene | Stab. Value | |
1 | ACTB | 0.061 | GAPDH | 0.053 | HPRT | 0.051 | GAPDH | 0.019 | GAPDH | 0.019 |
2 | GAPDH | 0.063 | ACTB | 0.053 | β2M | 0.053 | β2M | 0.034 | HPRT | 0.045 |
3 | β2M | 0.103 | 18S | 0.069 | ACTB | 0.062 | ACTB | 0.051 | β2M | 0.048 |
4 | HPRT | 0.105 | HPRT | 0.087 | GAPDH | 0.076 | HPRT | 0.053 | ACTB | 0.077 |
5 | 18S | 0.149 | β2M | 0.102 | 18S | 0.133 | 18S | 0.089 | 18S | 0.124 |
Ranking of candidate HKGs by NormFinder for each brain structure and all structures together, as described by the total M-value. Lower stability values indicate more stable genes. A cut-off value of 0.15 was used.