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. 2014 Oct 7;9(10):e110101. doi: 10.1371/journal.pone.0110101

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© 2014 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) ADP down-regulated the mRNA levels of Bcl-2. HUVEC were treated with indicated concentrations of ADP for 48 h. The mRNA levels of Bcl-2 was detected by RT-PCR. β-actin transcript was detected as loading control. (B, C) ADP down-regulated Bcl-2 protein expression in a dose- (B) and time-dependent (C) manner. (D) The effect of 2-MeSADP on Bcl-2 protein expression. (E) The effect of MRS2211 on Bcl-2 protein expression. (F) The effect of ADP pre-treatment on cisplatin-induced Bcl-2 down-regulation in HUVEC. Cells, pre-treated with the indicated concentrations of ADP for 48 h. were re-treated with 5 µM cisplatin for 48 h. Bcl-2 protein expression was detected by western blot. β-actin protein was detected as a loading control. (G) The effect of ADP pre-treatment on cisplatin-induced Bcl-2 protein expression in HAEC. (H) The effect of ADP pre-treatment on cisplatin-induced cell death. HUVEC were treated as same as (F). The cell survival was detected by CCK-8 assay. * P<0.05 compared with the control group. # P<0.05 compared with cisplatin-treated alone group.