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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Transplantation. 2014 Oct 15;98(7):721–728. doi: 10.1097/TP.0000000000000316

Figure 4. Activation of Nrf2 regulates Akt/Foxo1 signaling and TLR4-driven innate immune activation in vitro.

Figure 4

Murine BMM cultures were supplemented for 4h with with LPS (100ng/ml) +/− CoPP (50μM). (A) Western-assisted expression of Nrf2, HO-1, p-Akt, Foxo1, TLR4 and NF-κB. Representative of three experiments. (B–C) Quantitative RT-PCR-assisted detection of mRNA coding for TNF-α and IL-1β. Data were normalized to HPRT gene expression. Mean±SD; n=3–4/group; *p<0.05, **p<0.005.

Symbols used: Cells ( Inline graphic ); LPS ( Inline graphic ); CoPP+LPS ( Inline graphic ); Nrf2 KO+LPS ( Inline graphic ); Nrf2 KO+CoPP+LPS ( Inline graphic ).