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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Transplantation. 2014 Oct 15;98(7):721–728. doi: 10.1097/TP.0000000000000316

Figure 5. Inhibition of PI3K disrupts Akt/Foxo1 signaling and enhances TLR4-innate activation in vitro.

Figure 5

BMMs from WT mice were pretreated with PI3K inhibitor LY294002 or DMSO, followed by LPS +/− CoPP. (A) Western-assisted expression of Nrf2, p-Akt, Foxo1, TLR4 and NF-κB. β-actin served as an internal control. Representative of three experiments. (B–E) Quantitative RT-PCR-assisted detection of TNF-α, IL-1β, MCP-1 and CXCL-10. Mean�SD; n=3–4/group; *p<0.05, **p<0.005.

Symbols used: Cells ( Inline graphic ); LPS ( Inline graphic ); DMSO+CoPP+LPS ( Inline graphic ); LY294002+CoPP +LPS ( Inline graphic ).