Table 1.

Calibration Linearity

Nominal (ng/mL)	GM (ng/mL)	SD (ng/mL)	DEV (%)	CV (%)	n
0.50	0.51	0.06	2.75	5.50	8
1	0.97	0.05	−3.00	5.59	8
2	1.92	0.15	−4.25	7.94	8
5	5.03	0.49	0.62	9.84	8
25	25.41	1.14	1.63	4.49	8
50	50.70	5.00	1.39	9.87	8
100	103.73	7.39	3.73	7.12	8
500	499.48	25.66	−0.10	5.14	8

Abbreviations: GM, grand mean; SD, standard deviation; DEV (%), relative deviation from nominal value; CV (%), coefficient of variation; n, number of replicate observations within each validation run, i.e. two samples at each concentration were run on four separate occasions, for a total (n) of eight samples at each concentration.