Skip to main content
. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Aug 11;1842(11):2276–2285. doi: 10.1016/j.bbadis.2014.08.001

Figure 2.

Figure 2

TNFα and IL-1β mRNA production is reduced in the MyD88−/− microglia in response to protofibrils. SEC-isolated Aβ(1–42) protofibrils were incubated with WT primary microglia and MyD88−/− (KO) microglia at a final concentration of 15 µM for 2 hrs in serum-free medium. After 2 hrs the total RNA was collected and TNFα (Panel A) and IL-1β (Panel B) mRNA levels were quantified by qPCR as described in Fig. 1 legend. Data bars represent the mean ± std error of n=8 qPCR replicates. Protofibril-treated microglia were compared to aCSF-treated microglia to determine relative quantity. When comparing WT and MyD88−/− microglia, experiments were always done on the same day. Statistical analysis confirmed a significant difference (*p<0.001) between the WT and MyD88−/− microglial TNFα and IL-1β mRNA response.