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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Aug 11;1842(11):2276–2285. doi: 10.1016/j.bbadis.2014.08.001

Figure 3.

Figure 3

Aβ(1–42) protofibrils stimulate intracellular pro-IL-1β production in microglia. SEC-isolated Aβ(1–42) protofibrils in aCSF (gray bars) or aCSF buffer alone (black bars) were incubated with WT and MyD88−/− (KO) primary microglia at a final concentration of 15 µM for 6 hrs in serum-free medium. After incubation the conditioned medium was removed, each cell well was treated with 100 µL of lysis buffer and the extract was collected to measure intracellular pro-IL-1β protein by ELISA. Data bars represent the average ± std error of n=5 replicates. One-way ANOVA looking at WT and KO microglia individually showed a significant difference between control and protofibril-stimulated WT microglia (p<0.005) with no statistical difference in the KO microglia. Univariate ANOVA considering both treatment and the presence of MyD88 showed a lesser, but still significant, difference (*p<0.05) between the WT and KO microglial response.