Skip to main content
. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Aug 11;1842(11):2276–2285. doi: 10.1016/j.bbadis.2014.08.001

Figure 8.

Figure 8

Aβ protofibril-induced rapid IL-1β secretion is not sustained but is repeatable. Panel A. Primary microglia were exposed to SEC-isolated Aβ(1–42) protofibrils (15 µM) and allowed to incubate at 37 °C for 1 hr. The conditioned medium was then removed, fresh medium applied, and collected at 2 hr. This process was repeated again for the 4 hr analysis. No further IL-1β was secreted compared to the 1 hr time point (p<0.001). Panel B. Experiments were conducted as described in Panel A, although Aβ(1–42) protofibrils (15 µM) were added with the fresh medium at 1 and 2 hrs. Data bars represent the average ± std error of n=5 replicates. No statistical difference (NS) was noted in the amount of secreted IL-1β elicited by each successive Aβ(1–42) profibril treatment (p>0.05).