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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Aug 29;1841(11):1596–1607. doi: 10.1016/j.bbalip.2014.08.015

Fig. 7. The effect of dietary methionine deficiency on liver pathology.

Fig. 7

Male C57BL/6NCr wild-type mice at 8–12 weeks of age were treated with MCD (designated as MD+ CD+), choline-deficient diet (CD, designated as MD− CD+), methonine-deficient diet (MD, designated as MD+ CD−), or control MCS diet (designated as MD− CD−) for two weeks (n = 4–7/group) and serum and liver were collected.

(A) Body weight (BW) change. Values were expressed as the percentage relative to BW just before commencing the MCD, CD, MD, or MCS treatment.

(B) Liver TG contents.

(C–E) Serum levels of glucose (C), FGF21 (D), and ALT (E).

(F) Hepatic Tnf mRNA levels. The mRNA levels were normalized to those of 18S ribosomal mRNA and subsequently normalized to those of MCS-treated mice.

(G) Representative liver histology. Hematoxylin and eosin staining, Bar = 100 μm. Arrows in MCD photograph indicate inflammatory foci.

Statistical analysis was performed using the Student’s t-test. #P<0.05, ##P<0.01, ###P<0.001 vs. MCS-treated mice (MD− CD−); *P<0.05, **P<0.01, ***P<0.001 vs. CD-treated mice (MD− CD+); NS, not significant.