Fig. 7.

Chi-dependent protease cleavage is reduced in a nuclease-deficient mutant RecBCD enzyme but is enhanced in the absence of RecD. (a) RecBCD enzyme or RecB^D1080ACD enzyme was reacted in the absence of DNA or during reaction with Chi⁰ or Chi⁺ DNA and treated with 32 nM trypsin. Additional data are in Fig. S2. (b) RecBCD enzyme or RecBC enzyme (50 nM in ME buffer) was digested for 1 min with trypsin at the indicated concentrations. Reaction and sample preparation were as in Fig. 2.