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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Cell Calcium. 2014 Aug 4;56(4):245–256. doi: 10.1016/j.ceca.2014.07.012

Figure 2.

Figure 2

Activation of the mouse olfactory receptor mOR-EG transiently co-expressed either with Gα15 or Gαolf proteins. Note the cognate agonist eugenol (100μM) activates both the Gα15-dependent (red trace) and Gαs(olf)-dependent (blue) metabotropic signaling pathways. For comparison, similar expression of the heteromeric mosquito A. gambiae OR (AgOR65+AgOrco) resulted in a much faster response to the cognate agonist eugenol (1μM, light grey trace) reflecting direct Ca2+ influx through the ionotropic OR. Traces represent normalized averaged response to a 5-s stimulation recorded in a single experiment from 20-30 different cells. Stimulus was always triggered at 14.7 s time point. Three parameters of the response were measured: time of peak (Tpeak, a time of peak since stimulus onset), rise time (10% to 90%, Trise), and decay time (90% to 10%, Tdecay).