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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Cell Calcium. 2014 Aug 4;56(4):245–256. doi: 10.1016/j.ceca.2014.07.012

Figure 6.

Figure 6

Methyl isoeugenol acts as an antagonist on mOR-EG independent of the heterologous system. (A) Even at the highest concentration of 1mM methyl isoeugenol did not evoke any appreciable Ca2+ signal in Gα15 (red) or Gαs(olf)-expressing cells (blue). (B) Adding 1mM methyl isoeugenol (MIEG) completely eliminated Ca2+ response evoked by 100 μM eugenol. Control response to 1mM eugenol is shown for reference (shaded box). At least three independent experiments of this kind were performed. Each data point is the mean ΔF/F ± SD (grey bars) of fluorescence intensity of individual mOR-EG/Gα15 cells (red) and mOR-EG/Gαs(olf) cells (blue).