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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Cell Calcium. 2014 Aug 4;56(4):245–256. doi: 10.1016/j.ceca.2014.07.012

Figure 8.

Figure 8

Ligand binding to the mOREG activates both signaling pathways simultaneously in the same cell in a ligand-induced selective manner. HEK293T cells were co-transfected with mOR-EG, Gα15, Gαs(olf) and CNGC-camp. Representative recordings of each type of the response measured in nine different cells are shown in each panel. Application of isoeugenol (left, open panel) and eugenol (right, shaded panel) evoked in the majority of cells either (A) Gα15- or (B) Gαs(olf)-dependent response. (C) However, a small but significant fraction of cells generated a biphasic response with the initial fast rising phase associated with the Gα15-dependent pathway followed by a slower Gαs(olf)-dependent Ca2+ signal. The cell response patterns are consistent throughout the range of concentrations. Notably even saturating concentration of a putative selective mOR-EG ligand isoeugenol failed to evoke noticeable Gαs(olf)-dependent Ca2+ signal. The response of each cell was normalized to the respective maximum ΔF/F.