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. Author manuscript; available in PMC: 2015 Sep 12.
Published in final edited form as: Oncogene. 2014 Apr 7;34(11):1463–1474. doi: 10.1038/onc.2014.34

Fig. 1. Elevated expression of LNK in various cancers and serum-starved cells.

Fig. 1

A. LNK expression in various human cancers compared to matched normal tissue. Left panel, LNK mRNA levels higher in the normal tissue v/s the cancer samples. Right panel, LNK mRNA levels higher in the cancer samples v/s normal tissues. Data are from GEO database. B. LNK expression in five different molecular subtypes 31 of ovarian cancer patients samples (left panel) and ovarian cancer cell lines (right panel). C. Kaplan-Meier plots of overall survival of 316 ovarian cancer patients: comparison of cases with high levels (EXP:>2, defined as: > mean + 2SD, left panel) versus low levels (EXP <-2, defined as: < mean - 2SD, right panel) of LNK mRNA. P values are calculated by log rank test and indicated on the graph. Data are obtained from TCGA (The Cancer Genome Atlas) ovarian cancer project using the cBio Cancer Genomics Portal. D. IHC staining of ovarian cancer tissue array stained with LNK antibody (Photo of the whole section core of each sample are provide in Supplemental Fig 4 and which appear in the same order): #1, Normal ovary 1; #2, Normal ovary 2; #3, Benign ovarian tumor; #4, Clear cell ovarian carcinoma; #5, Serous Cystadenocarcinoma of the ovary (Grade I); #6, Serous cystadenocarcinoma (Grade II); #7, Serous cystadenocarcinoma (Grade III); #8, Serous cystadenocarcinoma (Grade III); #9, Endometrioid carcinoma of the ovary (Grade I), #10, Endometrioid carcinoma (Grade II) #11, Endometrioid carcinoma (Grade III), #12, Endometrioid carcinoma (Grade III). E. IHC staining score of 84 ovarian samples (see Supplementary Material for scoring intensity). F. Western blots of endogenous LNK levels in ovarian cancer cell lines (A2008 and C13) and melanoma cell lines (M285 and M368) during serum starvation (18 hours, SS) and followed by addition of serum (2 hours). Confluent cultures were changed to serum-free medium for 18 hours, and then switched back to complete medium containing 10% FBS for 2 hours. Western blot was performed to examine the LNK protein at indicated time points. Ctrl (control cell grown in the complete medium containing 10% FBS). G Western blot of LNK in the ovarian cancer cell line OVCA433 with forced expressed LNK. Cells were serum-starved (18 hours) followed by serum stimulation (as described above) for 1–3 hours, and LNK protein was examined by western blot. β-actin was used as loading control.