Figure 4.
NMDAR EPSPs are not eliminated in slices pretreated with low ketamine. In the presence of CNQX and low Mg2+ NMDAR EPSPs were reliably recorded in ketamine pretreated slices (N=5). For these studies, slices were pretreated with 10 µM ketamine for 30 min and ketamine was washed out for 2 hours prior to recording. In these slices, administration of 10 µM ifenprodil (gray bar) partially depressed NMDAR EPSPs and addition of 5 µM D-APV (black bar) almost completely depressed NMDAR EPSPs in a reversible manner. Traces show representative waveforms at designated times with the initial control traces as thin lines. Calibration: 1 mV, 5 msec.