Table 1.

List of primers used in the study of the seasonal relationships of gonadotropin, growth hormone, and estrogen receptor mRNA expression in the pituitary of wild largemouth bass. Note that primer sets are given in 5′ to 3′ direction. The real time PCR (qPCR) ER primers have previously been shown to be specific to each ER subtype (Sabo-Attwood et al., 2004).

	Forward 5′-3′	Reverse 5′ to 3′	base pairs
LHβ	CCA GAG AGG ATG ATG GCT GT	AAC CGT CAC CTA CCC CG	349
FSHβ	ACC CAG CAG AGG ATG CAG	CCT ACC CTG TGG CCA GAA	292
GTHα	CCA GCA TGG ACT TAT CAA ACA	GTA GCA ATT CTG TGT CTG TGA TGT	434
GH	GTC TGT TCT CCA TCG CTG TC	GAC GAA CCT ACG AAC TGC TG	452
qPCR
LHβ	TGC CAG CTC ATC AAC CAG	TGT CTT CCT GGC GTG GAC	207
FSHβ	CAT CTC ACC AAC ATC AGC ATC	ACG ACT GGG CTG AAC AGA A	131
GTH-α	CGA TCC CAA AGA ACA TCA CC	GGA AAG TGG AAA GTG GAG ACC	160
GH	GAG CAG CGT CAA CTC AAC AA	TTC TCT GTC TGG AGG TTC TGC	176
ERα	CGA CGT GCT GGA ACC AAT GAC AGA G	AGG AGG AAA ATC ATC AGT GAC CGG AGG T	71
ERβb	CCG ACA CCG CCG TGG TGG ACT C	TTG AGG CTC CCC TTG CCC CGC T	96
ERβa	GTG ACC CGT CTG TCC ACA	TCT CCT GCA CTG ACC CCA GA	84