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. Author manuscript; available in PMC: 2014 Oct 8.
Published in final edited form as: J Am Chem Soc. 2013 Aug 27;135(36):13433–13439. doi: 10.1021/ja4051026

Figure 4. Light-activation of EGFP transcription in HEK293T cells.

Figure 4

A) Cells were co-transfected with the PCKRS, PylT, T7RNApK631TAG and T7-IRES-EGFP constructs then incubated with 2 mM PCK for 24 hours. Irradiation was then performed for 2 minutes at 365 nm, and cells were imaged 48 hours after UV exposure. Scale bars indicate 200 μm. B) Fluorescent cell counting of caged T7RNAp-driven EGFP expression of cells 48 hours after UV exposure. The frequencies of EGFP-positive cells (gated/total) were normalized to the 10 minute UV irradiation and error bars represent the standard deviation obtained from three experimental replicates.