Table II. Putative surface-associated proteins detected in biotin-labelled adult worm whole body extracts that were absent from unlabelled controls. ESP, excretory-secretory products; AM, adult male; OG, octyl β-d-glucopyranoside; gAF, gravid adult female; FMN, flavin mononucleotide.

Parasite stage	Treatment	Peptides used for quantitation	Confidence score	Locus tag	Annotation	Presence in ESP
AM	OG	7	857.06	nLs_06907	Adenylate kinase isoenzyme 1	No
	OG	4	417.27	nLs_09715	Major sperm protein	Yes
	OG	2	186.68	nLs_01742	Filarial antigen Av33	No
	OG	2	297.13	nLs_08458	Filarial antigen Ov16	Yes
	SDS	2	308.12	nLs_07359	Calponin actin-binding domain containing protein	No
gAF	OG	2	233.80	nLs_09095	Protein disulphide isomerase	No
	SDS	2	86.22	nLs_08755	Leucine-rich repeat family protein	No
	SDS	3	118.56	nLs_09715	Major sperm protein	Yes
	SDS	2	99.43	nLs_02353	Complement component 1, q subcomponent-binding, mitochondrial-like	No
	SDS	2	61.97	nLs_01344	Thioredoxin peroxidase 1	No
	SDS	2	108.87	nLs_07321	Vitellogenin	Yes
	PBS	2	309.24	nLs_00851	DNA repair protein Rad4-containing protein	Yes
	PBS	2	309.24	nLs_07061	Heat shock 70 kDa protein	Yes
	PBS	2	309.24	nLs_09360	FMN-binding domain protein	No
	PBS	3	463.79	nLs_01364	Transthyretin-like protein, partial	Yes
	PBS	2	309.24	nLs_03263	Thioredoxin domain-containing protein	Yes