Fig. 1.

Experimental workflow to identify proteins whose phosphorylation and interaction with 14-3-3 proteins is modulated by dsRNA in human keratinocytes. A, samples were analyzed using phosphoproteomics and quantitative 14-3-3 affinity chromatography, and bioinformatic analyses were done with Ingenuity Pathway Analysis (IPA) and GeneTrail software. Details of experiments are described in “Experimental Procedures.” B, HaCaT keratinocytes were transfected with pI:C for the indicated times, and the expression of RIG-I, MDA5, and MAVS was analyzed from cell lysates via immunoblotting. GAPDH detection was used to confirm the equal loading.