Table I. Potential biomarker candidates chosen for verification by immunohistochemistry.

The adjusted p values for paired Student's t test (two-sided) and fold changes are given. All p values shown are corrected for multiple testing, controlling the false discovery rate. In Progenesis, p values are obtained using the method of Benjamini and Hochberg (21); DeCyder uses the version Benjamini and Hochberg published in 2000 (52). In the case of two values for t test and fold change for one protein, this corresponds to different isoforms identified by 2D-DIGE.

Number	Protein			2D-DIGE		Label-free proteomics
	Accession	Protein name	Gene name	Paired t test	Fold change	Paired t test	Fold change
Up-regulated in CCC
1	P06727	Apolipoprotein A-IV	APOA4	0.010	2.3	0.003	3.9
2	O00299	Chloride intracellular channel protein 1	CLIC1	0.009	4.7	0.007	2.4
3	P06396	Gelsolin	GSN	0.044	5.8	0.001	5.7
4	P26038	Moesin	MSN	0.021	2.5	–	–
5	P14618	Pyruvate kinase isozymes M1/M2	PKM2	0.025/0.047	8.8/2.6	<0.001	7.3
6	Q15181	Inorganic pyrophosphatase	PPA1	0.001/0.011	2.1/1.8	–	–
7	P50454	Serpin H1	SERPINH1	–	–	0.001	3.6
8	P31947	14-3-3 protein σ	SFN	–	–	0.01	8.2
9	P31948	Stress-induced phosphoprotein 1	STIP1	0.006	1.7	0.007	1.7
Down-regulated in CCC
10	P80404	4-aminobutyrate aminotransferase, mitochondrial	ABAT	0.001	5.4	<0.001	9.9
11	P42765	3-ketoacyl-CoA thiolase, mitochondrial	ACAA2	0.001/0.001	11.7/8.0	<0.001	8.7
12	Q93088	Betaine-homocysteine S-methyltransferase 1	BHMT	0.001/0.009	11.9/3.1	<0.001	17.1
13	P07148	Fatty acid–binding protein, liver	FABP1	0.001	30.5	<0.001	22.9
14	P54868	Hydroxymethylglutaryl-CoA synthase, mitochondrial	HMGCS2	0.016/0.032	2.7/1.8	<0.001	14.0