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Illustrative HCD, ETD, and EThcD MS2 spectra of an interpeptide disulfide bridge. The MS2 product ion spectra are reconstructed based on deconvoluted neutral monoisotopic masses. The two disulfide-cleaved fragment ions are labeled as peptides A and B. Single-cleavage ions (including b-, y-, c-, z- and b-, y-, c-, z- + A/B ions) are labeled as b-, y-, c-, z-ion series. Double-cleavage ions (including b-, y-, c, z- + S-S ions) are labeled as b*-, y*-, c*-, z*-ion series.
