Fig. 8.

A, structural view of a classical IgG1 antibody. Intrachain and interchain disulfide bridges are labeled as black and blue lines. Cysteines that are found to be involved in reshuffled disulfide bonds are shown in red. B, quantitative characterization of unambiguously identified disulfide-bridged peptides in non-heated and heated therapeutic antibodies. The authentic and reshuffled disulfide bonds are labeled in black and red, respectively. The inset shows an enlarged view of reshuffled disulfide bridges that were only observed in the heated samples.