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. 2014 Sep 28;13:225. doi: 10.1186/1476-4598-13-225

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© Sun et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Overexpression of THBS2 inhibited the proliferation of gastric cancer cells, suppressed the angiogenesis of gastric cancer, and promoted apoptosis in SGC-7901 cell line in vitro . (A) Western blot analysis of Flag in 293 T cells with THBS2 overexpression. (B) Colony number analysis for MKN-45 and SGC-7901 cells with THBS2 overexpression and negative control. Values are means ± SD from three independent experiments. (C) Images of colony formation assay. (D) AngiogenicIndexCh1 analysis of endothelial cell tube formation assay. Values are means ± SD. (E) Images of endothelial cell tube formation assay. (F) Analysis of apoptosis in MKN-45 cell line. Values are means ± SD.