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. 2014 Sep 24;14:90. doi: 10.1186/s12935-014-0090-z

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© Albawardi et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Metastatic colorectal adenocarcinoma. (a - f) Histology and expression of caspase-3 by immunoperoxidase. (a - b and c - d) Sample consisted of necrotic neoplastic tissue, H&E, 10× and 40×, respectively. (c and f) Caspase-3 demonstrated a positive staining (black arrow) in up to 5% of necrotic neoplastic cells in untreated tissue and absence of staining in treated tissue, immunoperoxidase (IP), 40×. (g - h) HPLC runs of intracellular caspase-3 activity in treated and untreated tumor (AMC peak retention time, ~4.8 min).