FIG. 4.

DNA capture capacity measurement. 160 μl of 1 ng/μl DNA solution is loaded, followed by 30 μl of washing buffer, and 70 μl elution buffer, all at a rate of 5 μl/min. The capture capacity of the 1 mm long monolith occupying 0.3 μl of channel volume is reached at approximately 110 ng and the concentration of DNA in the eluent rises to match the inlet concentration. After washing, approximately 40% of the captured DNA is eluted in a sharp peak with an elevated concentration.