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. 2014 Oct 8;5:513. doi: 10.3389/fmicb.2014.00513

Table 1.

Primers used in the present study.

Gene target Primer name Primer sequence (5′-3′) Amplicon size (bp) Reference
16S rDNA CIDIF-F CTTGAATATCAAAGGTGAGCCA 1085 Kikuchi et al. (2002)
CIDIF-R CTACAATCCGAACTGAGAGTA
tcdA tcdA-F3345 GCATGATAAGGCAACTTCAGTGGTA 629 Persson et al. (2008)
tcdA-R3969 AGTTCCTCCTGCTCCATCAAATG
tcdB tcdB-F5670 CCAAARTGGAGTGTTACAAACAGGTG 410 Persson et al. (2008)
tcdB-R6079A GCATTTCTCCATTCTCAGCAAAGTA
tcdB-R6079B GCATTTCTCCGTTTTCAGCAAAGTA
cdtA cdtA-F739A GGGAAGCACTATATTAAAGCAGAAGC 221 Persson et al. (2008)
cdtA-F739B GGGAAACATTATATTAAAGCAGAAGC
cdtA-R958 CTGGGTTAGGATTATTTACTGGACCA
cdtB cdtB-F617 TTGACCCAAAGTTGATGTCTGATTG 262 Persson et al. (2008)
cdtB-R878 CGGATCTCTTGCTTCAGTCTTTATAG
tcdA 3′-end deletions NK9 CCACCAGCTGCAGCCATA 2355a Kato et al. (1999)
NKV011 TTTTGATCCTATAGAATCTAACTTAGTAAC

aAmplicon size when no deletion was present.