Table 1.
Gene target | Primer name | Primer sequence (5′-3′) | Amplicon size (bp) | Reference |
---|---|---|---|---|
16S rDNA | CIDIF-F | CTTGAATATCAAAGGTGAGCCA | 1085 | Kikuchi et al. (2002) |
CIDIF-R | CTACAATCCGAACTGAGAGTA | |||
tcdA | tcdA-F3345 | GCATGATAAGGCAACTTCAGTGGTA | 629 | Persson et al. (2008) |
tcdA-R3969 | AGTTCCTCCTGCTCCATCAAATG | |||
tcdB | tcdB-F5670 | CCAAARTGGAGTGTTACAAACAGGTG | 410 | Persson et al. (2008) |
tcdB-R6079A | GCATTTCTCCATTCTCAGCAAAGTA | |||
tcdB-R6079B | GCATTTCTCCGTTTTCAGCAAAGTA | |||
cdtA | cdtA-F739A | GGGAAGCACTATATTAAAGCAGAAGC | 221 | Persson et al. (2008) |
cdtA-F739B | GGGAAACATTATATTAAAGCAGAAGC | |||
cdtA-R958 | CTGGGTTAGGATTATTTACTGGACCA | |||
cdtB | cdtB-F617 | TTGACCCAAAGTTGATGTCTGATTG | 262 | Persson et al. (2008) |
cdtB-R878 | CGGATCTCTTGCTTCAGTCTTTATAG | |||
tcdA 3′-end deletions | NK9 | CCACCAGCTGCAGCCATA | 2355a | Kato et al. (1999) |
NKV011 | TTTTGATCCTATAGAATCTAACTTAGTAAC | |||
aAmplicon size when no deletion was present.