Figure 1.

Characterization of the phenotype classes. (A) Representative plants of the four phenotype classes I–IV in the F₂ segregating population DM19 obtained from a cross between mutants yel and cola. From left to right: wild-type plant with green and normal leaf structure (I); yel-mutant (II); cola-mutant (III); yel/cola double mutant (IV) (Bar = 10 cm). (B) Morphology of reproductive organs. Left: epigynous flower of wild-type. Right: hypogynous flower of cola-mutant (Bars = 1.0 mm). (C) Histo-morphological sections of flowers. Left: wild-type. Right: cola-mutant. Black arrowheads: petals, yellow arrowheads: ovule (Bars = 0.5 mm). (D) FDA pollen staining. Fertile pollen grains revealed a intensive greenish stain, whereas sterile and undeveloped pollen grains were stained weakly; Left: wild-type, Right: cola-mutant, arrowheads indicate undeveloped microspores.