Table 1.

Primer sequences and amplification conditions for DcMADS3, DcMADS5, DcAOX2a, DcAOX2b, and DcCHS2.

Target	NCBI number	Marker type	Forward primer	Reverse primer	Annealing temperature	Fragments* detected in the
						yel—mutant	cola—mutant
DcMADS3	AJ271149	EST	GTGTGATGCTAAGGTTTCG	GATCCTGCTCCGCCATG	54	–	1500 bp
DcMADS5	AJ271151	CAPS**	GGGCACAAAGGAGCTTGAGG	AGAGCATCCACCCTGGAATG	50	605 bp	770 bp
DcAOX2a	EU286575	EST	TGCTGCATCTGAGGTCTCTCC	CCAATCAATTCTACACAACAACC	55	1900 bp	–
DcAOX2b	EU286576	EST	TGCATGCGTCCTTCCTTATTTTTC	AGCTTTGGTGACAGTATGTATAGG	55	–	1400 bp
DcCHS2	D16255	EST	CTCAAGGAGAAGTTTAGGCGGATG	ATGAGGCCATGTACTCGCAGAAA	56	850 bp	900 bp

^*Only polymorphic fragments were summarized. Fragments non-polymorphic between yel- and cola-parent were not mentioned.

^**The polymorphism was obtained after cleavage of the genomic DNA with Alu I to generate a CAPS (Cleaved Amplified Polymorphic Sequence) marker.