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. 2014 Oct 8;6:268. doi: 10.3389/fnagi.2014.00268

Figure 1.

Figure 1

Macroautophagy is predominantly responsible for α-syn degradation in 3D5 cells upon nutrient deprivation (ND). RA-elicited differentiated 3D5 cells bearing only endogenous α-syn, were respectively subjected to B27 deprived media with or without addition of different inhibitors MG-132, CQ, NH4Cl, 3-MA and WM for 24 h. The α-syn level in cells without nutrient deprivation (Con) was set as 100%, which was the initial level of α-syn for degradation in the following 24 h under ND. (A) The degradation of α-syn was evaluated and compared by western blotting of cell lysates. (B) Bar graph summarized the data from three independent experiments. Error bars represent standard error of the mean (* p < 0.05, ** p < 0.01, comparing to Con).