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. 2014 Oct 8;6:268. doi: 10.3389/fnagi.2014.00268

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Copyright © 2014 Jiang, Gan, Lin and Yen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nutrient deprivation induces accumulation of intracellular neutral lipids and cholesterol in 3D5 cells. 3D5 cells with RA-elicited differentiation and 10 days of TetOff induction were subjected to nutrient deprivation for 24 h. Sibling cell cultures without the deprivation were used as control. Cells were fixed in 4% paraformaldehyde and stained with (A) LipidTOX™ Green and Filipin for evaluation of neutral lipids and cholesterol, respectively. NLS: Neutral lipids staining; CS: Cholesterol Staining. Scale bar: 5 µm. (B,C) Results from quantitative image analysis using ImageJ demonstrated that nutrient deprivation induced significant increases of both intracellular neutral lipids (B) and cholesterol (C) contents. Error bars represent standard error of the mean (** p < 0.01, n = 3).