Fig. 2.
Protein levels of IκBα, p105, p50 and p65 in H. pylori-infected AGS cells treated with MAPK inhibitors. AGS cells were seeded in 12-well culture plates at 105 cells per well and cultured to reach 80% confluency. The MAP kinase inhibitors, U0126 (an ERK inhibitor) and SB203580 (a p38 inhibitor) (at 20 μM final concentration), were pretreated to the culture medium 1 h before the treatment of H. pylori. The bacterial cells were added to the cultured cells at a bacterium/cell ratio of 500:1 for 1 h (IκBα, A) and 2 h (p105, p50 and p65, B), respectively. None, AGS cells without treatment and cultured in the absence of H. pylori; Control, AGS cells without treatment and cultured in the presence of H. pylori; U0126, AGS cells treated with U0126 and cultured in the presence of H. pylori; SB203580, AGS cells treated with SB203580 and cultured in the presence of H. pylori.