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Dose-dependent effect of urushiol on the levels of cell cycle regulatory proteins in MKN-28 cells. MKN-28 cells were treated with the indicated concentration of urushiol for 48 h. Total cell lysates were prepared and the indicated protein levels were determined by Western blotting. β-actin was used as an internal loading control. A representative blot is shown from three independent experiments. p53 (m), mutant type p53.
